请使用支持JavaScript的浏览器!
主营:分子类,蛋白类,抗体类,生化类试剂
banner
当前位置: 首页 > 产品中心 > Actin-Toolkits > hypermol/Actin-Toolkit TIRFM (ATTO488-Actin)/8093-01

hypermol/Actin-Toolkit TIRFM (ATTO488-Actin)/8093-01

hypermol/Actin-Toolkit TIRFM (ATTO488-Actin)/8093-01
  • hypermol/Actin-Toolkit TIRFM (ATTO488-Actin)/8093-01
商品介绍
Description

Actin-Toolkit TIRFMThe complete solution!

ATTO488-Actin,rabbit skeletal muscle actinCat.# 8093-01

..............................................................................................................................................................................................................................................................................................................................................................................................................

Applications

- Single molecule analyses of actin polymerization, depolymerization and nucleation;

- Visualization of ligand-induced actin dynamics;

- High- and superresolution analysis of actin molecules, filaments, bundles and networks;

Description

Protein

ATTO488-Actin (α-Actin)

Origin

Skeletal muscle alpha actin, rabbit

Molecular mass

42kDa

Toolkit description

TheActin-ToolkitTIRFM was developed to analyze actin dynamics by TIRF microscopyor other fluorescence based single molecule imaging techniques. The Toolkit contains all components required for TIRFM analyses and enables the user to start TIRFM experiments straight ahead.

It includesATTO488-Actin (λex488nm,λem523nm) with a DOL of 0.3as demanded for TIRFM analysis.The Toolkit contains NEM-Myosin II as a tethering protein, and all components required for flow chamber TIRF assays, including FluMaXx -an efficient oxygen scavenger system -to protect actin filament from fragmentation during analyses. A detailed description of the flow chamber assembly and TIRFM actin polymerization assays is provided with the handbook.

In combination with the actinbufferscontained in thekit, error-free handling is guaranteed. The kit contains 5x100 µg ofα-skeletal muscle ATTO488-Actin conjugates and buffers to keep the actin functional.

Purity

>99%by scanning densitometry.

Properties

Form

Lyophilized and in solution (see kit content).

Kit content

5x100 µg ATTO488-Actin (lyophilized powder);1x100 µg NEM-Myosin II (lyophilized powder);3x1 ml Passivation solution (5% BSA, fatty acid free);1x1 ml PolyMix (lyophilized F-actin ATP-buffer);1x50 ml MonoMix (lyophilized G-actin ATP-buffer);1x10 ml HSB 5x stock (solution,Myosin Buffer, pH 7.0);1x0.5 ml ME-Buffer (100x Mg-Exchange buffer);1x Toolkit Handbook TIRFM;https://hypermol.com/gx/https://www.hypermol.com/images/Flumaxximg.png

+ FluMaXxOxygen Scavenger System for single molecule imaging(Cat. #: 5161-01)

Content

5x250 µlFluMaXx(2x stock, lyophilized) Contains: Glucose oxidase, catalase, imidazole pH 7.4, KCl, EGTA, MgCl2, glucose, cryoprotectants (proprietary formula);1x500 µl ATP/DTT Mix (10xstock, lyophilized)Contains: ATP, DTT2x1.5 ml Methylcellulose stock solution (0.5% in Milli-Q™ water)

Actin Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3and 0.3% disaccharides,when reconstituted with 1.0 mlultrapure water to obtain a 1mg/ml solution.

Related products

Actin Binding Proteins

Purification notes

Purified from rabbit skeletal muscle, GPC.

Protein concentration

Determined by the Biuret method.

Storage instructions

We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival.Solubilized G-actin is kept on ice and should be used within 1 week.Avoid refreezing.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

51005-14-2 (Actin)

Further Information

Material and Safety Data Sheet
Table of Dyes and Light SourcesProtein Sequence on NCBI
References

Modulationofactindynamicsaspotentialmacrophagesubtype-targetinganti-tumourstrategy.Pergola C, Schubert K, Pace S, Ziereisen J, Nikels F, Scherer O, Hüttel S, Zahler S, Vollmar AM, Weinigel C, Rummler S, Müller R, Raasch M, Mosig A, Koeberle A, Werz O.

Sci Rep. 2017 Jan 30;7:41434. doi: 10.1038/srep41434.

品牌介绍

hypermol是专业的肌动蛋白和肌动蛋白结合蛋白的纯化和修改专家,与全世界整合实验室和制药公司建立了良好的合作关系,除了提供定制蛋白质和缀合物的合成服务外,还生产和销售hypermol抗体和分析试剂


蚂蚁淘(ebiomall)作为一家生命科学领域的垂直电商平台,公司专注生物医学科研用品的全球导购和品牌推广,为海内外厂方与代理经销商搭建跨境贸易系统和交流平台。产品齐全,200+分类,1000+品牌,500万+产品信息。100%正品,专业售后,到货快,在线下单, 简单轻松,节省科学家宝贵的科研时间。为您竭诚服务!

【订购】蚂蚁淘生物科技  联系方式:4000-520-616

更多产品,更多优惠!请联系我们!

苏州蚂蚁淘生物科技有限公司

免费热线:4000-520-616

官网:https://www.ebiomall.cn/



Hypermol缓冲区服务的一些研究应用需要使用肌动蛋白和肌动蛋白配体的特定解决方案。
我们准备包含dH2O的特定缓冲液,或适用于EGTA / Mg ++的缓冲液-系统或脂质。
与所有产品一样,所有缓冲液均由分析纯化学品在超纯水中制备。  

自营商城图标
厂家直采
全球直采模式 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔
在线客服
客服电话

4000-520-616

0512-67156496