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hypermol/Actin-Toolkit ELISA (alpha skeletal muscle actin)/8121-01

hypermol/Actin-Toolkit ELISA (alpha skeletal muscle actin)/8121-01
  • hypermol/Actin-Toolkit ELISA (alpha skeletal muscle actin)/8121-01
商品介绍
Description

Actin-Toolkit ELISAα-Actin,rabbit skeletal muscleCat. #: 8121-01

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Applications

- Proof of ligand-actin interactions by ELISA with purified, semipurified or crude extracts; - Suited to determine ligand binding G- or F-actin;- Analysis of pharmacological compounds, proteins or lipids altering ligand-actin-interaction -All kit components are suitable for furtherin vitroapplications;

Description

Proteins

Biotin-Actin (biotinylated α-skeletal muscle actin)

Origin

Skeletal muscle alpha actin, rabbit

Molecular mass

42kDa

Toolkit description

The Actin-Toolkit ELISA is used to analyse ligand interactions with G-Actin or F-actin by enzyme linked immunosorbant assay. Purified proteins or other ligands, semi purified ligands or crude extracts can be used for the assays. Thus the Toolkit is suited for quantitative analyses or to determine actin-binding at various concentrations e.g. with crude extracts.Two types of ELISA assay are utilized in the assays:1. Direct ELISA.: the ligand is coated to the surface of the plate and either G-Biotin-actin or F-biotin actin is used to directly interact with the ligand. To detect Biotin-Actin if successfully bound to the coated ligand, either e.g. anti-biotin antibodies (HRP or alk. Phosphatase conjugated) or e.g. Streptavidin (HRP or alk. Phosphatase conjugated) can be used.

2. Sandwich ELISA.: the ligand is coated to the surface of the plate and either G-Biotin-actin or F-actin is used to directly interact with the ligand. To detect Actin successfully bound to the coated ligand the anti-actin antibody (clone 2G2) is used to be detected by secondary antibodies (mouse anti IgG ormouse anti IgM- HRP or alk. Phosphatase conjugated)is used.Anti-Actin is an IgM and thus able to efficiently bind to five actin. This results in an amplification of the signal obtained by secondary antibodies.

The kit contains Biotin-Actin, Actin and monoclonal anti-Actin, buffers for ELISA keeping actin functional and preventing denaturing of actin. A handbook introduces and guides through the experimental work with actin.

Purity

>99%by scanning densitometry.

Properties

Form

Lyophilized, ready-to-use.

Kit content

4x100µg Biotin-Actin (lyophilized powder);4x50ml G- ATP-buffer (lyophilized);1x200ml F- ATP-buffer;1x100mM ATP (lyophilized);1x500mM DTT (lyophilized)1x50µg monoclonal anti-Actin;1x50mg BSA (fatty-acid free);1xToolkit Handbook Actin-Toolkit ELISA;

Actin Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3and 0.3% disaccharides, when reconstituted with 1.0 mlultrapure water to obtain a 1mg/ml solution.

Related products

Purification notes

Purified from rabbit skeletal muscle, GPC.

Protein concentration

Determined by the Biuret method.

Storage instructions

We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival.Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

51005-14-2 (Actin)

Further InformationMaterial and Safety Data Sheet

Protein Sequence on NCBI
品牌介绍

hypermol是专业的肌动蛋白和肌动蛋白结合蛋白的纯化和修改专家,与全世界整合实验室和制药公司建立了良好的合作关系,除了提供定制蛋白质和缀合物的合成服务外,还生产和销售hypermol抗体和分析试剂

hypermol的ATTO标记的F(ab)片段属于新一代荧光抗体,其特征在于其出色的荧光强度和出色的光稳定性。抗小鼠IgG F(ab)ATTO647N具有很高的光稳定性和很强的荧光量子产率,因此特别适合于高分辨率显微检查。



F(ab)片段的缀合物比完整抗体缀合物更可取:


-对于空间上难以接近的细胞结构;

-由于Fab片段中不存在Fc区,防止与带有Fc受体的膜的相互作用;



规范


山羊抗小鼠IgG F(ab)ATTO647N(H + L),免疫荧光:1:300-1:500,标记度(DOL):> 1-3,未缀合染料≤总荧光的5%。 


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