请使用支持JavaScript的浏览器!
主营:分子类,蛋白类,抗体类,生化类试剂
banner
当前位置: 首页 > 产品中心 > Actin-Toolkits > hypermol/Actin-Toolkit Microinjection (ATTO565-Actin, skeletal muscle actin)/8061-01

hypermol/Actin-Toolkit Microinjection (ATTO565-Actin, skeletal muscle actin)/8061-01

hypermol/Actin-Toolkit Microinjection (ATTO565-Actin, skeletal muscle actin)/8061-01
  • hypermol/Actin-Toolkit Microinjection (ATTO565-Actin, skeletal muscle actin)/8061-01
商品介绍
Description

Actin-Toolkit MicroinjectionATTO565-Actin, Skeletal Muscle alpha-ActinCat. #: 8061-01 ............................................................................................................................................................................................................................................................................................................................................................................................................

Applications

  • Visualization of transfected actin binding proteins affecting the actin cytoskeleton;

  • Study of the effects by injection of pre-formed ligand-actin complexes;

  • Analysis of components or culturing conditions on the actin cytoskeleton;

  • Suitable for use with cell or tissue extracts in biochemical assays;

Description

Protein

ATTO565-Actin

Origin

Skeletal muscle alpha actin, rabbit

Molecular mass

42kDa

Toolkit description

The Actin-Toolkit Microinjection all-in-one kit contains rhodamine conjugatedα-actin to be microinjected into either normal or transfected cells. For this purpose purified actin was covalently labeled at Cys 374 with tetramethylrhodamine, leaving the polymerization properties of actin essentially unimpaired.TheActin-ToolkitMicroinjection contains 4x100µg ATTO565-Actin, rhodamine conjugated BSA as a reference, ATP-buffers suited for microinjection of actin and mini filter units for the convenient preparation of injectable actin. A handbook is provided with the kit, giving a detailed description of the use of actin for the microinjection technique for a safe and error-free handling of Rhodamine-Actin.

Purity

>99%by scanning densitometry.

Properties

Form

Lyophilized, ready-to-use.

Kit content

4x100µg ATTO565-Actin (lyophilized powder);1x50µg Rhodamine-BSA (lyophilized powder);5x50ml MonoMix .01 (lyophilized G-actin ATP-buffer with 0.01mM DTT);5x100µl Syringe Filter Units 0.22µM;1x Toolkit Handbook Microinjection;

Actin Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3and 0.3% disaccharides,when reconstituted with 100µlultrapure water to obtain a 1mg/ml solution.

DOL

>0.5

Purification notes

Purified from rabbit skeletal muscle, GPC.

DOL

Determined by the Biuret method.

Storage instructions

We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival.Solubilized G-actin is kept on ice and should be used within 1 week.Avoid refreezing.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

Further Information

Protein Sequence on NCBI

Material and Safety Data Sheet

品牌介绍

hypermol是专业的肌动蛋白和肌动蛋白结合蛋白的纯化和修改专家,与全世界整合实验室和制药公司建立了良好的合作关系,除了提供定制蛋白质和缀合物的合成服务外,还生产和销售hypermol抗体和分析试剂


蚂蚁淘(ebiomall)作为一家生命科学领域的垂直电商平台,公司专注生物医学科研用品的全球导购和品牌推广,为海内外厂方与代理经销商搭建跨境贸易系统和交流平台。产品齐全,200+分类,1000+品牌,500万+产品信息。100%正品,专业售后,到货快,在线下单, 简单轻松,节省科学家宝贵的科研时间。为您竭诚服务!

【订购】蚂蚁淘生物科技  联系方式:4000-520-616

更多产品,更多优惠!请联系我们!

苏州蚂蚁淘生物科技有限公司

免费热线:4000-520-616

官网:https://www.ebiomall.cn/



hypermol的ATTO标记的F(ab)片段属于新一代荧光抗体,其特征在于其出色的荧光强度和出色的光稳定性。抗小鼠IgG F(ab)ATTO647N具有很高的光稳定性和很强的荧光量子产率,因此特别适合于高分辨率显微检查。



F(ab)片段的缀合物比完整抗体缀合物更可取:


-对于空间上难以接近的细胞结构;

-由于Fab片段中不存在Fc区,防止与带有Fc受体的膜的相互作用;



规范


山羊抗小鼠IgG F(ab)ATTO647N(H + L),免疫荧光:1:300-1:500,标记度(DOL):> 1-3,未缀合染料≤总荧光的5%。 


自营商城图标
厂家直采
全球直采模式 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔
在线客服
客服电话

4000-520-616

0512-67156496