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hypermol/Actin-Toolkit SPR (alpha cardiac muscle actin)/8900-01

hypermol/Actin-Toolkit SPR (alpha cardiac muscle actin)/8900-01
  • hypermol/Actin-Toolkit SPR (alpha cardiac muscle actin)/8900-01
商品介绍
Description

Actin-Toolkit SPRα-actin from bovine cardiac muscle

Cat. #: 8900-01

..............................................................................................................................................................................................................................................................................................................................................................................................................

Applications

- Characterization of binding kinetics and dissociation constants of ligands binding to F-actin;

- Analysis of proteins, lipids or pharmacological compounds altering ligand-actin-interaction;

Description

Protein

Actin (α-cardiac muscle Actin)

Origin

Cardiac muscle alpha actin, bovine

Molecular mass

42kDa

Toolkit description

Binding of ligands to filamentous actin is used as a functional proof to state the biological activity of a ligand and characterizes the ligand to be an actin binding protein. Furthermore, the specific actin binding strongly indicates that the ligand is correctly folded and structurally intact.The Actin-Toolkit SPR provides a unique method to precisely analyze ligand-F-actin interactions by Surface Plasmon Resonance techniques on Streptavidin chips. To overcome the general problem in SPR- which is that the actin filaments clog the capillaries of the device -short actinfilaments with AnkerproteinTM on the fast polymerizing barbed ends are used for for coating. The actin filaments protrude from the surface of the chip and permit the introduction of ligands.After the actin filaments become attached to the surface of the chip, ligands can be introduced to investigate binding to F-actin.The Actin-Toolkit SPR was developed to analyze the actin-binding properties of ligands (native or recombinant proteins, recombinant fragments etc.) and to support the detection even of cryptic actin-binding sites. Further it is suitable to investigate effects of pharmacological compounds on ligand-actin complexes, as well as further proteins, lipids etc. toligand-actin complexes.The kit contains rabbit skeletal muscle actin(Cat. # 8010-03), AnkerproteinTM, FAB-EP, GAP-EP (SPR actin buffers for F-and G-actin).A handbook introduces and guides through the experimental work with actin in SPR.Please note that the user requires Streptavidin coated chips. The setup for the compounds to investigate has to be determined individually.

Purity

>99%by scanning densitometry.

Properties

Form

Lyophilized, ready-to-use.

Kit content

4x250µg Actin (lyophilized powder);4x10µg AnkerproteinTM(lyophilized powder);1x PolyMix (actin polymerization buffer, 10x);2x250 ml FAB-EP, liquid (ready-to-use);1x250 ml GAB-EP, liquid ( ready-to-use);1xToolkit Handbook SPR;

Buffers

FAB-P: 100mM Imidazol-Cl pH 7.4, 0.4mM ATP, 2mM CaCl2 and 0.0005% v/v Surfactant P20.GAB-P: 2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2 and 0.0005% v/v Surfactant P20.

Related products

For dilution of G-actin or exchange of ligand buffer into actin compatible buffer, MonoMix (Cat. #: 5100-0*) maybe used. In the absence of nucleators, the polymerization of G-actin is initiated by PolyMix (Cat. #: 5000-0*).

Purification notes

Purified from skeletal muscle, rabbit; GPC.

Protein concentration

Determined by the Biuret method.

Storage instructions

We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival.Solubilized G-actin is kept on ice and should be used within 1 week.Avoid refreezing.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

Further Information

Material and Safety Data Sheet

Protein Sequence on NCBIBiacoreTMis a trademark of GE healthcare.AnkerproteinTMis a trademark of Hypermol.
品牌介绍

hypermol是专业的肌动蛋白和肌动蛋白结合蛋白的纯化和修改专家,与全世界整合实验室和制药公司建立了良好的合作关系,除了提供定制蛋白质和缀合物的合成服务外,还生产和销售hypermol抗体和分析试剂


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hypermol的蛋白质染色和标记试剂盒用高度光稳定的Atto荧光鬼笔环肽快速染色单个肌动蛋白丝。非常适合于免疫荧光样品的两次或多次染色。 




对于单分子成像,Tornado-SMI TM  被开发用于快速和安全的蛋白质标记。 Tornado-SMI TM  具有以下荧光波长:550nm,550nm或646nm(绿色,橙色和深红色荧光)。  




 



否则,可以使用现成的且稳定期短的稳定成分作为添加剂。


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