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hypermol/FluMaXx (Oxygen scavenger for single molecule imaging)/5161-01

hypermol/FluMaXx (Oxygen scavenger for single molecule imaging)/5161-01
  • hypermol/FluMaXx (Oxygen scavenger for single molecule imaging)/5161-01
商品介绍
Description

FluMaXxOxygen scavenging system for singlefluorescentmolecule imaging.Cat. #: 5161-01

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ApplicationsFlow chamber protein interaction assays, all applications demanding strong antifade supplements, Suited for all types offluorescence microscopyand spectroscopical applications and methods:TIRFM, Epifluorescence microscopy (EFM), GSD, STED, dStorm etc.

Description

Product name

FluMaXx

Product description

FluMaXx is an fluorescence oxygen scavenging system to improve the stability of fluorescent dyes. The efficiency of FluMaXx is illustrated by its ability to prevent e.g. filamentous actin from fragmentation, which are extremely prone to photon induced chemical damage.FluMaXxcontains all vital components for high resolution monitoring of assays using cytoskeletal polymers.Thisoptimized catalase/glucose oxidase enzyme system, protects the natural fluorescence intensity of the dye and thus maXximizes the fluorescence intensity.A novel optimization of FluMaXx is based on the stabilization of the catalyse enzyme. Catalase normally cannot be frozen in solution without a major loss of activity and furthermore is inactivated by higher concentrations of reducing agents.Our proprietary formula of theFluMaXxpermits refreezing after reconstitution without loss of activity. The buffer contains 5mM DTT, showing a beneficial effect on performance without measurable damage of the enzyme system.To guarantee the high quality ofFluMaXx, all critical components are supplied as a lyophilized powder.

Overview

High performance oxygen scavenger to :

  • efficiently prolong fluorescence;
  • prevent protein damage;
  • prevent fragmentation of polymers;

Properties

Form

Lyophilized, three-component buffer system, ready-to-use.(Contains methylcellulose as 0.5% solution)

ComponentsFinal product volume

5x 500µlSol. A (lyophilized) Contains: Glucose oxidase, catalase, imidazole pH 7.4, KCl, EGTA, MgCl2, glucose, cryoprotectants (proprietary formula);1x 500µl Sol. B (lyophilized)Contains: ATP, DTT1x 1.5ml Methylcellulose stock solution (0.5% in Milli-Q™ water)2.5ml (100-200 assays)

Working instructions

For flow chamber assays: add 250µl of ultrapure water to the tube labelled Sol. A and mix with 200µlof methylcellulose (MC).After addition of the MC-solution to Sol. A, the mixture is vortexed and centrifuged for 1min, 5000rpm.

Sol. B (ATP/DTT-Mix) is added freshly before mixing with the sample solution to be observed (e.g. 45µlSol. A/MC + 5µl Sol. B). It is recommended to shortly degas FluMaXx before use to remove trapped air from the viscous solution.As antifading buffer system: add 450µl of ultrapure water to the tubelabelled Sol. A. Mix Sol. B shortly before use(e.g. 45µlSol. A/MC + 5µl Sol. B).

Purity

All components were made up in Milli-Q™ water and0.2µm filtered.

Storage instructions

FluMaXxis stored at -70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. After reconstitution aliquots of FluMaXx,Sol. A/MC and Sol. B solutionscan be prepared for storage.

Store on ice after reconstitution and use final mixtures within 1 day.Prepare aliquots immediately after reconstitution for deep freezing.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use inHuman or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

Further Information

Material and Safety Data Sheet

References

Modulationofactindynamicsaspotentialmacrophagesubtype-targetinganti-tumourstrategy.Pergola C, Schubert K, Pace S, Ziereisen J, Nikels F, Scherer O, Hüttel S, Zahler S, Vollmar AM, Weinigel C, Rummler S, Müller R, Raasch M, Mosig A, Koeberle A, Werz O.

Sci Rep. 2017 Jan 30;7:41434. doi: 10.1038/srep41434.

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hypermol的FluoProtect是一种高效的防褪色嵌入介质,用于荧光显微镜,干燥时间为24小时。嵌入介质适用于荧光显微镜,并与所有已知的荧光染料兼容。它是一种带有附加防褪色剂的水性封固剂,可大大减少光漂白。

如果将样品保存避光,荧光将保留数周。与许多安装介质相比,FluoProtect在任何常见波长下均不显示自发荧光。

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