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hypermol/Actin (porcine skeletal muscle alpha-actin) - 1.0mg/8111-01

  • 产品编号: 8111-01
  • 美  元  价: $10400.00
  • 市  场  价: ¥208000.00
  • 品       牌: hypermol
  • 产       地: 美国(厂家直采)
  • 公       司: hypermol
  • 产品分类: 抗猪>二抗>抗体类>
  • 公司分类: Proteins
hypermol/Actin (porcine skeletal muscle alpha-actin) - 1.0mg/8111-01
  • hypermol/Actin (porcine skeletal muscle alpha-actin) - 1.0mg/8111-01
商品介绍
Description

Actinalpha-actin from porcine skeletal muscleCat. #: 8111-01

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Description

Protein

Actin (α-Actin)

Origin

Skeletal muscle alpha actin, porcine.

Molecular mass

42kDa

Protein description

Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids. It is characterized bybinding to ATP/ADP (ATPase function) and the binding sites for divalent cations, that strongly contribute to the stabilization of actin.Actin trulyis amulti-ligand protein, which simply reflects its central importanceas a major component of the cytoskeleton.The skeletal muscle actin (alpha-actin isoform) is highly conserved among vertebrates, which makes the rabbit skeletal muscle a commonlyused protein to investigate protein-protein interactions with cytoskeletal ligands.The ability of actin monomers e.g. topolymerize into filaments of several µm lengths, to branch or perform the typical actin kinetics,strongly depends on the quality of the actin.Hypermol ismanufacturinga premium quality actin. And to provide you the best actin we can possibly make, ourproprietaryfreeze-drying technique conserves highly functionalactin. to arrive directly at your bench.

Purity

>99%by scanning densitometry.

Properties

Form

Lyophilized, ready-to-use.

Quantity per unit

1 mg

Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3and 0.3% disaccharides, when reconstituted with 1.0 mlultrapure water to obtain a 1mg/ml solution.

Related products

For dilution of G-actin or exchange of ligand buffer into actin compatible buffer, MonoMix (Cat. #.: 5100-0*) maybe used. In the absence of nucleators, the polymerization of G-actin is initiated by PolyMix (Cat.#: 5000-0*).

Purification notes

Purified from porcine skeletal muscle. GPC.

Protein concentration

Determined by the Biuret method.

Storage instructions

Actin stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. Solubilized G-actin is kept on ice and should be used within 1 week.Avoid refreezing.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

51005-14-2

Further Information

Product DataSheetMaterial and Safety Data SheetProtein Sequence on NCBI

References

Cosolvent and crowding effects on the polymerization kinetics of actin.Rosin, Christopher, Paul Hendrik Schummel and Robert S. C. Winter.

Physical chemistry chemical physics : PCCP 17 13 (2015): 8330-7.

Exploringthestabilitylimitsofactinand itssuprastructures.Rosin C, Erlkamp M, Ecken JV, Raunser S, Winter R.

Biophys J. 2014 Dec 16;107(12):2982-2992. doi: 10.1016/j.bpj.2014.11.006.

Multivalentcross-linkingofactinfilamentsandmicrotubulesthrough themicrotubule-associatedproteinTau.Cabrales Fontela Y, Kadavath H, Biernat J, Riedel D, Mandelkow E, Zweckstetter M.

Nat Commun. 2017 Dec 7;8(1):1981. doi: 10.1038/s41467-017-02230-8.

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hypermol是专业的肌动蛋白和肌动蛋白结合蛋白的纯化和修改专家,与全世界整合实验室和制药公司建立了良好的合作关系,除了提供定制蛋白质和缀合物的合成服务外,还生产和销售hypermol抗体和分析试剂


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hypermol的ATTO标记的F(ab)片段属于新一代荧光抗体,其特征在于其出色的荧光强度和出色的光稳定性。抗小鼠IgG F(ab)ATTO647N具有很高的光稳定性和很强的荧光量子产率,因此特别适合于高分辨率显微检查。



F(ab)片段的缀合物比完整抗体缀合物更可取:


-对于空间上难以接近的细胞结构;

-由于Fab片段中不存在Fc区,防止与带有Fc受体的膜的相互作用;



规范


山羊抗小鼠IgG F(ab)ATTO647N(H + L),免疫荧光:1:300-1:500,标记度(DOL):> 1-3,未缀合染料≤总荧光的5%。 


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