hypermol/Alpha-Actinin (turkey gizzard smooth muscle) - 1.0 mg/8301-02

Protein

a-Actinin

Origin

Smooth muscle, turkey gizzard

Molecular mass

~200kDa

Protein description

Nativeα-actinin from smooth muscle has a molecular mass of 200kDa and forms homodimers (2x100kDa).

Actin interaction

Actin cross linking protein. Kd actin-binding = 1-5µM.

Form

Lyophilized, ready-to-use.

Quantity per unit

1x1 mg

Buffer

50mMNaCl, 20mM Tris pH 7.4, 0.5mM DTT and 3% disaccharides, when reconstituted with 1 ml ultrapure water to obtain a 1mg/ml solution.

Purity

>97%by scanning densitometryfrom Coomassie G-250 stained SDS-Gels.

Purification notes

Purified from turkey gizzard muscle(IEX, SEC).

Protein concentration

DeterminedUV-VIS (A₂₈₀=0.1201)

Storage instructions

Upon reconstitution the product is stored on ice. This product can also be stored as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

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