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hypermol/Alpha-Actinin (turkey gizzard smooth muscle) - 1.0 mg/8301-02

hypermol/Alpha-Actinin (turkey gizzard smooth muscle) - 1.0 mg/8301-02
  • hypermol/Alpha-Actinin (turkey gizzard smooth muscle) - 1.0 mg/8301-02
商品介绍
Description

α-Actinin(Actin Binding Protein)Cat. #: 8301-02

.....................................................................................................................................................................................................................................................................................................................................................................................................................................................

Description

Protein

a-Actinin

Origin

Smooth muscle, turkey gizzard

Molecular mass

~200kDa

Protein description

Nativeα-actinin from smooth muscle has a molecular mass of 200kDa and forms homodimers (2x100kDa).

Actin interaction

Actin cross linking protein. Kd actin-binding = 1-5µM.

Properties

Form

Lyophilized, ready-to-use.

Quantity per unit

1x1 mg

Buffer

50mMNaCl, 20mM Tris pH 7.4, 0.5mM DTT and 3% disaccharides, when reconstituted with 1 ml ultrapure water to obtain a 1mg/ml solution.

Purity

>97%by scanning densitometryfrom Coomassie G-250 stained SDS-Gels.

Purification notes

Purified from turkey gizzard muscle(IEX, SEC).

Protein concentration

DeterminedUV-VIS (A280=0.1201)

Storage instructions

Upon reconstitution the product is stored on ice. This product can also be stored as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

Further Information

Product DataSheetMaterial and Safety Data SheetProtein Sequence on NCBI (chain A)Protein Sequence on NCBI (chain B)

ReferencesReal-timedynamicsofemergingactinnetworksincell-mimickingcompartments.Deshpande S, Pfohl T.;​PLoS One. 2015 Mar 18;10(3):e0116521. doi: 10.1371/journal.pone.0116521. eCollection 2015.

A high molecular mass protein isolated from chicken gizzard: its localization at the dense plaques and dense bodies of smooth muscle and the Z-disks of skeletal muscle.Terasaki AG, Nakagawa H, Kotani E, Mori H, Ohashi K.;J Cell Sci. 1995 Mar;108 ( Pt 3):857-68

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