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当前位置: 首页 > 产品中心 > Proteins > hypermol/ATTO647-Actin for TIRFM (alpha-Actin, skeletal muscle rabbit) - 2x100µg/8158-03

hypermol/ATTO647-Actin for TIRFM (alpha-Actin, skeletal muscle rabbit) - 2x100µg/8158-03

hypermol/ATTO647-Actin for TIRFM (alpha-Actin, skeletal muscle rabbit) - 2x100µg/8158-03
  • hypermol/ATTO647-Actin for TIRFM (alpha-Actin, skeletal muscle rabbit) - 2x100µg/8158-03
商品介绍
Description

Atto647-Actin for TIRFMα-actin from skeletal muscle, rabbitCat. #: 8158-03

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Description

Protein

Atto647-Actin

Origin

Skeletal muscle alpha actin, rabbit

Molecular mass

42kDa

Protein description

Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids. Atto647-Actin is chemically modified G-actin with an Atto647 fluorescent group covalently bound to lysine residues.Atto647-Actin(λex647nm,λem669nm)possesses the polymerization properties of native G-actin.

Conjugation ratio

This product is supplied with a degree of labelling (DOL) of ~0.3, suited for TIRF microscopy.

Properties

Form

Lyophilized, ready-to-use.

Quantity per unit

2x100 µg

Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3and 0.3% disaccharides, when reconstituted with100µl ultrapure H2Oto obtain a 1mg/ml solution.

Purity

>99%by scanning densitometry.

Purification notes

Purified from rabbit skeletal muscle.

Protein concentration

Determined by the Biuret method.

Storage instructions

Atto647-Actin stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized productis kept on ice, protect from light and it should be used within 1 week.Avoid refreezing of actin.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

Further Information

Product DataSheet

Material and Safety Data SheetTable of Dyes and Light SourcesProtein Sequence on NCBI

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Hypermol缓冲区服务的一些研究应用需要使用肌动蛋白和肌动蛋白配体的特定解决方案。
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