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hypermol/ATTO514-Actin for TIRFM (non-muscle) - 2x50µg/8354-03

hypermol/ATTO514-Actin for TIRFM (non-muscle) - 2x50µg/8354-03
  • hypermol/ATTO514-Actin for TIRFM (non-muscle) - 2x50µg/8354-03
商品介绍
Description

ATTO514-Actin for TIRFMNon-muscle actin from brain, porcine

Cat. #: 8354-03

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Description

Protein

ATTO514-Actin

Origin

Non-muscle β/γfrom brain, porcine

Molecular mass

42kDa

Protein description

Actin from rnon-miscle sources are a mixture ofβ/γnon-muscle actins. These isoforms are single chain polypeptides with a molecular weight of 42kD consisting of 375 amino acids.ATTO514-Actin is chemically modified G-actin with an ATTO514 fluorescent group covalently bound to lysine residues.ATTO514-Actin (λex511nm, λem533nm)possesses the polymerization properties of native G-actin.

Conjugation ratio

This product is supplied with a degree of labelling (DOL) of ~ 0.3 and is thus ready-to-use for TIRFM and other high-or super-resolution imaging techniques..

Properties

Form

Lyophilized, ready-to-use.

Quantityper unit

2x50µg

Buffer

2.0mM Tris-Cl pH 8.2, 1.0mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3and 0.3% disaccharides, when reconstituted with 100 µl ultrapure water to obtain a 1mg/ml solution.

Purity

>99%by scanning densitometry.

Purification notes

Purified by GPC from rabbit skeletal muscle.

Protein concentration

Determined by the Biuret method.

Storage instructions

ATTO514-Actin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. SolubilizedATTO-Actinis kept on ice and should be used within 1 week. Avoid refreezing.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

Further Information

Product DataSheetTable of Dyes and Light Sources Protein Sequence on NCBIMaterial and Safety Data Sheet

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hypermol的蛋白质染色和标记试剂盒用高度光稳定的Atto荧光鬼笔环肽快速染色单个肌动蛋白丝。非常适合于免疫荧光样品的两次或多次染色。 




对于单分子成像,Tornado-SMI TM  被开发用于快速和安全的蛋白质标记。 Tornado-SMI TM  具有以下荧光波长:550nm,550nm或646nm(绿色,橙色和深红色荧光)。  




 



否则,可以使用现成的且稳定期短的稳定成分作为添加剂。


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