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hypermol/ATTO565-Actin for TIRFM (non-muscle actin) - 2x50µg/8356-03

hypermol/ATTO565-Actin for TIRFM (non-muscle actin) - 2x50µg/8356-03
  • hypermol/ATTO565-Actin for TIRFM (non-muscle actin) - 2x50µg/8356-03
商品介绍
Description

ATTO565-Actin for TIRFMNon-muscle actin from brain, porcineCat. #: 8356-03

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Description

Protein

ATTO565-Actin

Origin

Non-muscle β/γactin, porcine

Molecular mass

42kDa

Protein description

Actin from non-muscle sources is a mixture of β/γnon-muscle actin isoforms. Both isoforms aresingle chain polypeptides with a molecular weight of 42kD consisting of 375 amino acids. ATTO565-Actin is chemically modified G-actin with an ATTO565 fluorescent group covalently bound to lysine residues.ATTO565-Actin(λex563nm,λem592nm)possesses the polymerization properties of native G-actin.

Conjugation ratio

This product is supplied with a degree of labelling (DOL) of ~0.3 and is thus ready-to-use for TIRFM and other high- or super-resolution timaging echniques.

Properties

Form

Lyophilized, ready-to-use.

Quantity per unit

2x50 µg

Buffer

2.0mM Tris-Cl pH 8.2, 1.0mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3and 0.3% disaccharides, when reconstituted with 100 µlultrapure water to obtain a 1mg/ml solution.

Purity

>99%by scanning densitometry.

Purification notes

Purified by GPC from porcine brain.

Protein concentration

Determined by the Biuret method.

Storage instructions

ATTO565-Actin stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized productis kept on ice, protect from light and it should be used within 1 week.Avoid refreezing of actin.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

Further Information

Product DataSheetMaterial and Safety Data SheetTable of Dyes and Light SourcesProtein Sequence on NCBI

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hypermol的二级抗体的质量对免疫荧光产生良好结果至关重要。为检测高质量的原代抗体提供了新的、高质量的抗体。




Atto标记抗体属于新一代荧光抗体,具有荧光强度高、光稳定性好等特点。因此,它们适用于各种免疫荧光显微术,也主要用于高分辨率技术。




与已建立的荧光抗体相比,由于染料的突出特性,Atto抗体显示出显著延长的荧光,如两个例子所示:




1ATTO647N在水溶液中的荧光强度是Cy5TM的两倍。


2在相同的条件下,ATTO647N比CY5T和Alexa647TM等染料的使用寿命长100倍。




用紫外可见分光光度法和免疫细胞化学法对金丝桃中的Atto结合igg进行亲和纯化和质量控制。它们是一种方便的试剂盒,具有良好的特性,表位映射肌动蛋白抗体,供参考,双重染色或配体分析,每个试剂盒的产品文档中都有详细的描述


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