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hypermol/Talin (smooth muscle, turkey) - 100µg/8314-01

hypermol/Talin (smooth muscle, turkey) - 100µg/8314-01
  • hypermol/Talin (smooth muscle, turkey) - 100µg/8314-01
商品介绍
Description

Talin(Actin Binding Protein)Cat. #: 8314-01

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Description

Protein

Talin (Talin-1)

Origin

Smooth muscle, turkey

Molecular mass

~270kDa

Protein description

Talin isolated from smooth muscle has a molecular mass of ~270kD. Talin consists of a 220kD rod containing actin, vinculin and integrin binding sites and an N-terminal 47kD head region with a FERM domain. The actin-binding multiligand protein Talin interacts e.g. with phospholipids, signaling molecules, and cytoskeletal components.

Actin interaction

Actin binding protein.

Properties

Form

Lyophilized, ready-to-use.

Quantity per unit

100 µg

Buffer

0.1M KCl, 10mM imidazole pH 7.4, 2mM MgCl2, 0.2mM DTT and 2% disaccharides, when reconstituted with 100µlultrapure water to obtain a 1mg/ml solution.

Purity

~94%by scanning densitometryfrom Coomassie G-250 stained SDS-Gels.

Purification notes

Purified from turkey gizzard muscle.

Protein concentration

Determinedby Biuret method.

Storage instructions

Upon reconstitution the product is stored on ice. This product can also be stored as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

Further Information

Product DataSheet
Material and Safety Data SheetProtein Sequence on NCBI

Literature

A high molecular mass protein isolated from chicken gizzard: its localization at the dense plaques and dense bodies of smooth muscle and the Z-disks of skeletal muscle.

Terasaki AG, Nakagawa H, Kotani E, Mori H, Ohashi K.J Cell Sci. 1995 Mar;108 ( Pt 3):857-68

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hypermol是专业的肌动蛋白和肌动蛋白结合蛋白的纯化和修改专家,与全世界整合实验室和制药公司建立了良好的合作关系,除了提供定制蛋白质和缀合物的合成服务外,还生产和销售hypermol抗体和分析试剂


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Hypermol的缓冲液由分析纯化学品在超纯水中制成,可以满足您的高要求。 

在这里,您可以找到具有最佳保质期的短寿命成分(ATP,DTT)的即用型缓冲液。 
例如,肌动蛋白聚合缓冲液PolyMix 是一步一步提供的10倍储备溶液:添加1ml超纯水,并与G-肌动蛋白以1:10 v / v混合。PolyMix用于稀释F-肌动蛋白和配体,提供满足肌动蛋白丝体外工作条件的条件  

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