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hypermol/Filamin (smooth muscle, turkey) - 2x100 µg/8312-01

  • 产品编号: 8312-01
  • 美  元  价: $29800.00
  • 市  场  价: ¥596000.00
  • 品       牌: hypermol
  • 产       地: 美国(厂家直采)
  • 公       司: hypermol
  • 产品分类: >耗材>其它>
  • 公司分类: Proteins
hypermol/Filamin (smooth muscle, turkey) - 2x100 µg/8312-01
  • hypermol/Filamin (smooth muscle, turkey) - 2x100 µg/8312-01
商品介绍
Description

Filamin(Actin Binding Protein)Cat. #: 8312-01

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Description

Protein

Filamin (Filamin A)

Origin

Smooth muscle, turkey

Molecular mass

546kDa

Protein description

Filamin from turkey gizzard is a homodimer (2x273kDa) with a molecular mass of 546kDa, consisting of 24 repeats of ~96 amino acids and an N-terminal actin binding sequence. Filamin crosslinks actin filaments into orthogonal networks.The C-terminal end of each Filamin monomer is characterized by a hinge region, responsible for the flexibility of actin networks.

Actin interaction

Actin cross linking protein. Kd actin-binding = 0.5µM.

Properties

Form

Lyophilized, ready-to-use.

Quantity per unit

2x100µg

Buffer

10mMNaCl, 20mM Tris pH 7.4, 0.5mM DTT and 2.4% disaccharides, when reconstituted with 100 µlultrapure water to obtain a 1mg/ml solution.

Purity

>98%by scanning densitometryfrom Coomassie G-250 stained SDS-Gels.

Purification notes

Purified from turkey gizzard muscle.

Protein concentration

Determinedby OD280 (0.1%=0.64).

Storage instructions

Upon reconstitution the product is stored on ice. This product can also be stored as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

Further Information

Product DataSheetMaterial and Safety Data SheetProtein Sequence on NCBI

ReferencesReal-timedynamicsofemergingactinnetworksincell-mimickingcompartments.Deshpande S, Pfohl T.; ​PLoS One. 2015 Mar 18;10(3):e0116521. doi: 10.1371/journal.pone.0116521. eCollection 2015.

A high molecular mass protein isolated from chicken gizzard: its localization at the dense plaques and dense bodies of smooth muscle and the Z-disks of skeletal muscle.Terasaki AG, Nakagawa H, Kotani E, Mori H, Ohashi K.,J Cell Sci. 1995 Mar;108 ( Pt 3):857-68

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hypermol是专业的肌动蛋白和肌动蛋白结合蛋白的纯化和修改专家,与全世界整合实验室和制药公司建立了良好的合作关系,除了提供定制蛋白质和缀合物的合成服务外,还生产和销售hypermol抗体和分析试剂


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hypermol的二级抗体的质量对免疫荧光产生良好结果至关重要。为检测高质量的原代抗体提供了新的、高质量的抗体。




Atto标记抗体属于新一代荧光抗体,具有荧光强度高、光稳定性好等特点。因此,它们适用于各种免疫荧光显微术,也主要用于高分辨率技术。




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1ATTO647N在水溶液中的荧光强度是Cy5TM的两倍。


2在相同的条件下,ATTO647N比CY5T和Alexa647TM等染料的使用寿命长100倍。




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