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hypermol/ATPase Pi (ATPase Assay)/Quantity/6061-01

hypermol/ATPase Pi (ATPase Assay)/Quantity/6061-01
  • hypermol/ATPase Pi (ATPase Assay)/Quantity/6061-01
商品介绍
Description

ATPase PiKit for determination of ATPase activities.Cat. #: 6061-01

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Description

Product name

ATPase Pi (ATPase Assay)

Product description

The ATPase Piis used to determine the enzymatic activity of e.g. myosins, S1, HMM and actomyosin, by measuring the inorganic phosphate released upon ATP-hydrolysisThe ATPase Piassay is ideally suited to determine the ATPase activity myosins and its proteolytic fragments, as it is reliably easy-to-handle due to its moderate sensitivity range (50µM-1mM inorganic phosphate). Highly sensitive determination methods are usually too sensitive to give precise and reliable results for these proteins. ATPase Piis a modified assay based on the method of Fiske & Subbarow, where a bluish phosphomolybdate complex forms under reducing conditions. Since organic phosphates remain unrecognized, the assay is suited for phosphoproteins and nucleotides.

Overview

  • highly reproducible results;
  • stable components;
  • easy to handle;

Properties

Form

Powder. Ready-to-use upon addition of components (see below).

ComponentsQuantity

1.Solution M – 1x 100ml Molybdate Solution (add 100ml 2.6N sulphuric acid)

2.Solution R – 1x100ml Reduction Solution (add 100ml H2O ultrapure)

3.Phosphate-Standard (50ml 1mM KPO4 – ready-to-use)

4.Standardized solutions in H2O ultrapure: 1M imidazole pH 7.0, 3M KCl, 0.1M

MgCl2, 0.1M CaCl2, 0.1M EDTA pH 7.0, 0.1M ATP pH 7.0

Additional components required: TCA- trichloroacetic acid (50% solution)

Please note that we do not ship dangerous liquids. Thus these components are to be added following the laboratory safety instructions to protect your health. You are welcome to contact us for submission of the MSDSs for the substances to be added.~2000 assays

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hypermol是专业的肌动蛋白和肌动蛋白结合蛋白的纯化和修改专家,与全世界整合实验室和制药公司建立了良好的合作关系,除了提供定制蛋白质和缀合物的合成服务外,还生产和销售hypermol抗体和分析试剂


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如果将样品保存避光,荧光将保留数周。与许多安装介质相比,FluoProtect在任何常见波长下均不显示自发荧光。

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